ABSTRACT
Objective:To establish the identification method for Aucklandia Lappa and Picrorhiza kurrooa Royle ex Benth and the content determination of hydroxysaffor yellow A in Liangxue Shiwei San. Methods:The identification was carried out by TLC. The con-tent of hydroxysaffor yellow A was determined by HPLC. The column was Kormasil C18 (250 mm × 4. 6 mm, 5 μm) and the flow rate was 1. 0 ml·min-1. The mobile phase was methanol-0. 5% acetic acid (30∶70). The detection wavelength was 403 nm and the col-umn temperature was 25℃,and the sample size was 10 μl. Results:The TLC spots were clear with high resolution without interference from the negative sample. A good linearity of hydroxysaffor yellow A was within the range of 1.212-48.480μg·ml-1(r=0.999 8). The average recovery was 98. 13%(RSD=1. 6%,n=6). Conclusion:The established TLC and HPLC methods are simple and accu-rate with good reproducibility, which can be used in the quality control of Liangxue Shiwei San.
ABSTRACT
BACKGROUND:The dental pulp stem cells,marrow mesenchymal stem cells and periodontal ligament stem cells belong to the adult stem cells from oral cavity,which own two features of the adult stem calls:the ability of renewing themselves strongly and the potential of splitting.Through the continuous study of many kinds of stem cells from oral cavity,it is very helpful for human to show the process of making the firm organizations of tooth and the inducing mechanism,in order to direct the related clinical work and the prevention work.OBJECTIVE:To summarize features and application perspective of various adult stem cells from oral cavity in the clinic.METHODS:The first author retrieved PubMed database(http://www.ncbi.nlm.nih.gov/PubMed)and China National Knowledge Infrastructure(http://www.cnki.net)for relevant literatures published from January 1999 to December 2009.The key words were "stem call,dentistry".A total of 80 literatures were screened,and studies on basic research and clinical application of stem calls in stomatology medical field were included.Old and duplicated studies were excluded and finally 19 literatures were included.RESULTS AND CONCLUSION:With development of study,adult stem cells in oral cavity will be gradually found to establish integrated stem cell database,which can provide cell source for tissue engineering study in the oral cavity.